南美白对虾肝胰腺蛋白提取方法优化及无水保活环境胁迫对细胞凋亡蛋白的影响

为提高Western Blotting结果的可靠性，以蛋白溶出率和凝胶电泳蛋白条带完整性为指标，比较提取液种类、研磨方式、酶抑制剂种类及其体积分数和提取时间对南美白对虾肝胰腺蛋白提取效果的影响。采用优化后的提取方法获得高质量蛋白样品，并采用Western Blotting法分析无水环境胁迫后南美白对虾肝胰腺组织中细胞凋亡信号通路相关蛋白的表达水平。结果表明：RIPA裂解液作为提取溶剂所得的蛋白溶出率高于水提和磷酸盐缓冲液，电动匀浆和液氮研磨所得蛋白条带更完整，4%蛋白酶磷酸酶混合抑制剂能有效抑制肝胰腺内源酶引起的蛋白降解；采用Western Blotting法分析无水保活期间南美白对虾肝胰腺蛋白，发现低温诱导休眠的同时会引起细胞轻微凋亡，且凋亡水平呈应激时间依赖性增加，环境胁迫解除后有所回调。     

Environmental Enrichment Enhances Cav 2.1 Channel-Mediated Presynaptic Plasticity in Hypoxic–Ischemic Encephalopathy

Hypoxic–ischemic encephalopathy (HIE) is a devastating neonatal brain condition caused by lack of oxygen and limited blood flow. Environmental enrichment (EE) is a classic paradigm with a complex stimulation of physical, cognitive, and social components. EE can exert neuroplasticity and neuroprotective effects in immature brains. However, the exact mechanism of EE on the chronic condition of HIE remains unclear. HIE was induced by a permanent ligation of the right carotid artery, followed by an 8% O₂ hypoxic condition for 1 h. At 6 weeks of age, HIE mice were randomly assigned to either standard cages or EE cages. In the behavioral assessments, EE mice showed significantly improved motor performances in rotarod tests, ladder walking tests, and hanging wire tests, compared with HIE control mice. EE mice also significantly enhanced cognitive performances in Y-maze tests. Particularly, EE mice showed a significant increase in Ca_v 2.1 (P/Q type) and presynaptic proteins by molecular assessments, and a significant increase of Ca_v 2.1 in histological assessments of the cerebral cortex and hippocampus. These results indicate that EE can upregulate the expression of the Ca_v 2.1 channel and presynaptic proteins related to the synaptic vesicle cycle and neurotransmitter release, which may be responsible for motor and cognitive improvements in HIE.     

Novel cobalt-free Pr2Ni1-xNbxO4 (x = 0, 0.05, 0.10, and 0.15) perovskite as the cathode material for IT-SOFC

The cobalt-free cathode materials with high activity is critical to the commercial application of medium temperature solid oxide fuel cells (IT-SOFC). Herein, a series of cobalt-free Nb-doped Pr₂Ni_1-xNb_xO₄ (x = 0, 0.05, 0.10, and 0.15) perovskite oxides were successfully prepared, and the effects of Nb-doping on the structure, thermal stability and electrochemical properties of the cathode material are studied in detail. The Pr₂Ni_1-xNb_xO₄ exhibits a K₂NiF₄ type structure with Fmmm space group. The Nb⁵⁺ cations that doped into Pr₂NiO₄ replaces the Ni site, which increases the surface oxygen content, then effectively eliminates the phase transition of Pr₂NiO₄ and significantly improves the ORR catalytic activity. The Pr₂Ni_0.9Nb_0.1O₄ was found to occupy the lowest polarization resistance of 0.057 Ω cm², and the peak power density of single cells supported by the electrolyte is 0.576 W cm^?2 at 700 °C, which has good long-term stability.     

Functional properties and biological activities of perilla seed meal protein hydrolysates obtained by using different proteolytic enzymes

Food Science and Biotechnology - In this study, we aimed to determine the potential functional properties and biological activities of the hydrolysates of perilla seed meal (PSM), which is a...     

A cost effective on-site fault diagnosis method for home appliance rotor failures

Microsystem Technologies - Rotating components are one of the most important machine parts used in many industrial applications. Rotating machine commonly used in homes has a washing machine, which...     

Grouting of large karstic caverns in dobrogea Coalfield,Bulgaria

The paper describes the results of an experimental grouting programme carried out by the Spetstamponazhgeologia association at the Dobrogea coalfield in Bulgaria. The anticipated water inflow into a shaft at this deposit totals 8 m³/s at a pressure of 12 MPa, from a cavity size equal or exceeding 3 metres. Grouting operations made it possible to cut down water inflow by a factor of more than one thousand. The success of this investigation has enabled the author to design an exprrimental shaft sinking project through the valange formation at Dobrogea mine.     

对劳动力派遣的认识

劳动力派遣在现实生活中确实有一些负面影响,但它毕竟是市场经济中出现的一种企业用人、社会管理服务的新用工模式,而且有进一步发展的趋势.应尊重现实生活的需要,以法律加以规范,使其有序发展.     

基于 DII 建筑钢结构专利技术国际态势分析

基于DII专利数据库,利用TDA等专利分析工具和平台,对建筑钢结构技术国内外申请专利进行分析。研究结果表明:建筑钢结构技术相关专利是从2007年之后申请量开始快速增长,其中2000年以来申请的专利占全部专利的88.15%。专利申请排名前三位的国家依次为中国、日本和韩国。从研发热点来看,建筑钢结构专利技术申请主要集中在条形建筑构件结构、金属支承构件、防振动或震动的构件等方面。     

Electrical properties and the role of inhomogeneities at the polyvinyl alcohol/n‐inp schottky barrier interface

In this work, we have investigated the electrical properties of Au/n‐InP contacts with a thin layer of polyvinyl alcohol (PVA) as an interlayer. The current–voltage (I–V) and capacitance–voltage (C–V) measurements are carried out in the temperature range of 175–425 K. The Au/PVA/n‐InP Schottky structure show nonideal behaviors and indicates the presence of a nonuniform distribution of interface states. The temperature dependent interface states densities (N_SS), ideality factor and barrier height are obtained. An abnormal decrease in zero‐bias barrier height (BH) and increase in the ideality factor ( ) with decreasing temperature have been explained on the basis of the thermionic emission theory with Gaussian distribution (GD) of the BHs due to the BH inhomogeneities. The experimental I–V characteristics of Au/PVA/n‐InP Schottky diode has revealed the existence of a double GD with mean BH values of ( ) of 1.246 and 0.899 eV and standard deviation ( ) of 0.176 and 0.137 V, respectively. Consequently, the modified conventional activation energy versus plot gives and Richardson constants ( ) and the values are 1.17 and 0.71 eV and 9.9 and 6.9 A/cm² K², respectively, without using the temperature coefficient of the BH. The effective Richardson constant value of 9.9 A/cm² K² is very close to the theoretical value of 9.4 A/cm² K² for n‐InP. The discrepancy between Schottky barrier heights estimated from I–V and C–V measurements is also discussed. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 39773.     

单轴压缩下饱水裂隙砂岩体积效应的试验研究

为研究水和岩样体积对砂岩物理力学及变形特性的影响,采用MTS815型岩石力学测试系统,对干燥和饱水状态下的3种不同体积(高径比2∶ 1)完整和裂隙砂岩进行单轴压缩试验。结果表明:完整和裂隙砂岩的孔隙率与饱和吸水率与岩样体积成正比;干燥和饱水的完整与裂隙砂岩单轴抗压强度均随体积增大而小幅降低;砂岩弹性模量和变形模量与岩样体积呈负相关,且水降低了砂岩的弹性模量与变形模量;干燥砂岩的泊松比随岩样体积增加而增大,而饱水砂岩则随岩样体积先增加,后稳定;干燥的完整和裂隙砂岩的宏观破坏形式由小尺寸的轴向多劈裂面破坏逐渐转变为大尺寸的单一剪切破坏,水的存在对岩样单轴压缩破坏形态没有明显影响。